BHK21CL13-2P cell line (ECACC catalogue no. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies).
Species
Hamster
Tissue
Kidney
Morphology
Fibroblast
Passage Number
+13
Sub Culture Routine
Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37�C. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Culture Medium
InVitrus medium (Cell Culture Technologies) + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.
Karyotype
Not Specified
Depositor
Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.
Originator
Yes
Country
Switzerland
References
None Specified By Depositor
Additional Literature Report
Not Available
Additional Bibliography
Not Available
Research Council Deposit
No
Release Conditions
No
DNA Available from Stock
No
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details
distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where
quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore
further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and
ECACC does not guarantee the passage number stated will be the passage number received by the customer.
Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
