HT1080 cells serially transfected with synthetic HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector and amphotropic envelope protein from a MLV vector. These cells stably express synthetic HIV Gag-Pol, HIV tat, HIV rev sequences and amphotropic envelope protein. These cells serve as a packaging cell line for HIV based vectors.
Species
Human
Tissue
Fibrosarcoma
Morphology
Epithelial
Passage Number
+4
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3-5x10,000 cells/cm� using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37�C.
Professor Mary Collins, Department of Immunology and Molecular Pathology, University College London
Originator
Yes
Country
UK
References
Ikeda Y et. al., Nat. Biotechnol. (2003) May: 21(5): 569-572
Additional Literature Report
Not Available
Additional Bibliography
Not Available
Research Council Deposit
No
Release Conditions
Yes
DNA Available from Stock
No
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details
distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where
quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore
further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and
ECACC does not guarantee the passage number stated will be the passage number received by the customer.
Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
