22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta-1). This cell line is tumorigenic in nude mice.
Species
Human
Tissue
Prostate
Morphology
Epithelial
Passage Number
14
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm� using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37�C.
Culture Medium
RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
James W. Jacobberger, Case Western Reserve University, Cleveland, Ohio, USA
Originator
Yes
Country
USA
References
Sramkoski et al.: A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999.
Additional Literature Report
Not Available
Additional Bibliography
Not Available
Research Council Deposit
No
Release Conditions
No
DNA Available from Stock
No
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details
distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where
quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore
further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and
ECACC does not guarantee the passage number stated will be the passage number received by the customer.
Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
