General Cell Collection ECACC No. 05121902 Cell Line Name EasyCeL-PLC/PRF/5 Keywords EasyCeL Tube Format: Human liver hepatoma, Alexander cell line Cell Line Description Growing PLC/PRF/5 cells are supplied in cell culture tubes ready for use in clinical virology for the detection and isolation of viruses in clinical samples. Contact ECACC for more information about the EasyCeL format. Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Species Human Tissue Liver Morphology Epithelial Sub Culture Routine Cell lines supplied growing in tubes are designed for single use only. Subculture is not required. On arrival incubate tubes overnight at 37C; 5%CO2. Examine cells microscopically for attachment before use. Culture Medium Transport medium: DMEM + 2mM Glutamine + 1% Foetal Bovine Serum (FBS) + 100units/ml Penicillin + 0.1mg/ml Streptomycin + 2.5ug/ml Amphotericin. Karyotype Not specified Products Hepatitis B Surface Antigen (HBSAg) Depositor Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford Originator No Country UK References Proc Natl Acad Sci, USA 1985;82:83; J Virol 1979;32:796; Science 1980;209:497; Science 1983;222:385; Nature 1979;282:615 Additional Literature Report Not Available Additional Bibliography Not Available Research Council Deposit No Release Conditions No DNA Available from Stock No The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.   Delivery State Price Code - T Tubes -  �1.30     Quantity Required    Prices & Ordering Click the 'UPDATE' button to refresh the price for the quantity required. Click the Basket button to add this product to your order.    Net Total           �1.30